INVESTIGATION ON PHYTOCHEMICALS, ORAL ACUTE TOXICITY, AND PROTECTIVE EFFECT OF AQUEOUS EXTRACT OF AG-S90 POLYHERBAL FORMULATION ON ALLOXAN-INDUCED DIABETIC NEUROPATHY PAIN IN WISTAR RATS

Abstract

The use of natural product as formulated phytomedicine in Nigeria has significantly increased for mitigating the problem of emerging and re-emerging diseases such as Adayi Gambari Salihu-90 (AG-S90) polyherbal drug. No scientific data-based information, no scientific validation, and enough evidence available on this product for disease management. Aims: The study was aimed at determining phytochemicals, oral acute toxicity and to evaluate the neuroprotective effect of AG-S90 on Alloxan-induced diabetic neuropathic pain model in Wistar rats. Methods: Phytochemical screening was conducted according to standard methods, where acute oral toxicity study was performedfollowing OECD guidelines 425, using UP-and-DOWNprocedure, 2014 and evaluation of neuroprotective effect of AG-S90 against Alloxan-induced diabetic neuropathy in wistar rats weighing between 150-180 kg p.b.w. Experimental diabetes was induced in Wistar albino rats by single intraperitoneal injection of Alloxan monohydrate (150 mg/kg). Results: result indicated the presence of phytochemical (Alkaloids, Anthraquinones, Anthocyanins, Amino acids/proteins, Carbohydrates, Cardiac glycosides, Saponins, Flavonoids, Polyphenols, Tannins, Terpenoids, Steroids, Fats, and oils). Observational studies revealed normal activities without any behavioural abnormality. There is no death recorded nor any sign of toxicity in acute toxicity after oral administration. Conclusion: Aqueous extract of AG-S90 could be employed as a pain reliever, reversed diabetes to explore its various medicinal activity and very safe for human consumption (at LD50 greater than 5000mg/kg). It is concluded that AG-S90 exhibits rich phytochemicals, significant neuroprotective and lowered blood sugar levels (p<0.05) against diabetic neuropathy in rats.Findings, there was an improvement in diabetic state after AG-S90 treatment could be used to mops-up ROS, heals pancreatic gland and good therapeutic candidate for diabetic neuropathic pain.